An acceptor-dependent polyglycerolphosphate polymerase.

نویسندگان

  • J Mauck
  • L Glaser
چکیده

A method for the extraction of polyglycerolphosphate polymerase from Bacillus subtilis membranes is described. Further purification by ion-exchange chromatography yields an enzyme fraction totally dependent on the addition of a heat-stable factor for activity. This factor was isolated from cell membranes and acts as an acceptor of glycerolphosphate units. The acceptor contains glycerolphosphate, glucosamine, and fatty acids, but its precise chemical structure has not yet been elucidated.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

The Synthesis of Polyribitol Phosphate

The synthesis of polyribitol phosphate by polyribitol phosphate polymerase from Staphylococcus aureus H proceeds by a single chain mechanism so that the enzyme completes a chain of approximately 30 units linked to lipoteichoic acid carrier, before starting a new chain. The lipoteichoic acid carrier of Staphylococcus aweus and Bacillus subtilis are interchangeable and can be used both for the sy...

متن کامل

The Synthesis of Polyribitol Phosphate

The synthesis of polyribitol phosphate by polyribitol phosphate polymerase from Staphylococcus aureus H proceeds by a single chain mechanism so that the enzyme completes a chain of approximately 30 units linked to lipoteichoic acid carrier, before starting a new chain. The lipoteichoic acid carrier of Staphylococcus aweus and Bacillus subtilis are interchangeable and can be used both for the sy...

متن کامل

Two-enzyme systems for glycolipid and polyglycerolphosphate lipoteichoic acid synthesis in Listeria monocytogenes

Lipoteichoic acid (LTA) is an important cell wall polymer in gram-positive bacteria and often consists a polyglycerolphosphate backbone chain that is linked to the membrane by a glycolipid. In Listeria monocytogenes this glycolipid is Gal-Glc-DAG or Gal-Ptd-6Glc-DAG. Using a bioinformatics approach, we have identified L. monocytogenes genes predicted to be involved in glycolipid (lmo2555 and lm...

متن کامل

Bovine coronavirus 5'-proximal genomic acceptor hotspot for discontinuous transcription is 65 nucleotides wide.

Coronaviruses are positive-strand, RNA-dependent RNA polymerase-utilizing viruses that require a polymerase template switch, characterized as discontinuous transcription, to place a 5'-terminal genomic leader onto subgenomic mRNAs (sgmRNAs). The usually precise switch is thought to occur during the synthesis of negative-strand templates for sgmRNA production and to be directed by heptameric cor...

متن کامل

Inhibition of antibody-dependent stimulation of lipoteichoic acid-treated human monocytes and macrophages by polyglycerolphosphate-reactive peptides.

By itself, lipoteichoic acid (LTA) obtained from S. pyogenes, S. aureus, or E. hirae poorly stimulated cytokine production by macrophages, whereas in the presence of anti-polyglycerol phosphate (PGP), the cells secreted significant amounts of IL-6. Two peptides constructed from the deduced sequence of the selected anti-PGP phage-antibody's complementary-determining region 3 of the variable heav...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Proceedings of the National Academy of Sciences of the United States of America

دوره 69 9  شماره 

صفحات  -

تاریخ انتشار 1972